Ming-Na Wen has won multiple awards throughout her career, including an Annie Award and a Saturn Award, in addition to a nomination for a Screen Actors Guild Award. She was honored as a Disney Legend in 2019 and a star on the Hollywood Walk of Fame in 2023.

After gaining attention playing Lien Hughes on the daytime soap As the World Turns (1988–1991), Wen’s breakthrough role was as June Woo in The Joy Luck Club (1993). Other early successes include the role of Dr. Jing-Mei "Deb" Chen in the medical drama series ER (1995–2004) and her voice role as Fa Mulan in the Walt Disney Animated Classic Mulan, as well as its sequel Mulan II, several video games and guest appearances in TV shows such as Sofia the First. Additionally, Wen made a cameo appearance in the live-action remake of Mulan (2020).

Wen is well-known for playing agent Melinda May in the Marvel series Agents of S.H.I.E.L.D. (2013–2020) and has also appeared in the Star Wars franchise as the bounty hunter Fennec Shand, appearing in The Mandalorian (2019–2020), Star Wars: The Bad Batch (2021, 2024), and The Book of Boba Fett (2021–2022). Other notable roles include that of Chun-Li in Street Fighter (1994), Detective Ellen Yin in The Batman (2004–2005), Judge Linda Harris in Two and a Half Men (2007, 2010) and Camile Wray in Stargate Universe (2009–2011).